FLIm and Raman Spectroscopy for Investigating Biochemical Changes of Bovine Pericardium upon Genipin Cross-Linking

Anticalcification effects of decellularization, solvent, and detoxification treatment for genipin and glutaraldehyde fixation of bovine pericardium.

OBJECTIVE Calcification plays a major role in the failure of bioprosthetic and other tissue heart valve substitutes. The objective of this study was to evaluate the anticalcification effect of decellularization and detoxification in glutaraldehyde (GA)/genipin- and solvent-fixed bovine pericardium using a rabbit intramuscular model which is effective for assessing calcification in bioprosthetic...

High Performance Method for Skin Roughness Detection Using Raman Spectroscopy

Protein conformation changes of HemAT-Bs upon ligand binding probed by ultraviolet resonance Raman spectroscopy.

HemAT from Bacillus subtilis (HemAT-Bs) is a heme-based O2 sensor protein that acts as a signal transducer responsible for aerotaxis. HemAT-Bs discriminates its physiological effector (O2) from other gas molecules (CO and NO), although all of them bind to a heme. To monitor the conformational changes in the protein moiety upon binding of different ligands, we have investigated ultraviolet reson...

Structural changes of silicon upon high-energy milling investigated by Raman spectroscopy

This study showed pronounced changes in the Raman scattering of silicon powder during high-energy ball milling. The powders were milled for 1–18 h in a steel ball mill in argon. The approximate pressure imposed on particles was 2 GPa. The spectra of the as-milled powders were compared with the initial silicon. It was found from the Raman peak position shifts that milling generated strains in th...

Effects of genipin cross-linking of chitosan hydrogels on cellular adhesion and viability.

PURPOSE The aim of the present study was to investigate the effects of genipin (Gp) cross-linking of chitosan (CHI) hydrogels on the cell adhesion and viability. METHOD Series of Gp crosslinked CHI hydrogels were prepared by incubation of solutions containing a mixture of Gp and CHI in different ratios. The resulting hydrogels were characterized by scanning electron microscopy (SEM), parallel...